Palabras Clave: Aplicaciones del alginato, composición del alginato, fibras de to prepare moulds in dentistry (in presence of slow release calcium salt) Jeltrate® Alginate is indicated for making dental impressions for the fabrication of casts for: • case study models .. COMPOSICIÓN. Sílice cristalina . El tiempo de fraguado del alginato Jeltrate® Fraguado Normal es de aproximadamente. Alginato de sódio é um composto químico, é o sal de sódio do ácido algínico. Sua fórmula química empírica é NaC6H7O6. Ele forma uma goma, quando.
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El objetivo de este estudio fue evaluar la citotoxicidad de los alginatos para aplicaciones dentales. Fueron evaluados 14 alginatos diferentes: Alginate fel an impression material classified as an irreversible hydrocolloid that is easy to handle, allowing good detail reproducibility in addition to being cheap and comfortable for the patient Anusavice, Therefore, such a material is largely used in dentistry. However, despite its easy manipulation, perfect dental impressions using alginate are not always achieved by non-experienced students, thus often requiring repeated procedures Samuel et al.
Alginatos by Dario Sosa on Prezi
Amongst these metals, one can cite the lead, which is present in the alginate powders to improve their elastic properties following gelification despite sometimes being found as an impurity Braga et al. Basically, intoxication with alginate occurs through inhalation of the powder by patient and practitioner, accidental intake by the patient, and absorption by oral mucosa in the cases of repeated impression procedures Braga et al.
During the impression procedure, alginate is left in close contact with the oral mucosa for approximately 2 minutes, and this tissue is highly vascularised and has great absorption potential. Therefore, repeated impression procedures might cause a certain degree of cytotoxicity to the patient depending on the material composition Braga et al.
Alginates differ from each other according to the components present in their formulation.
Based on this premise, the objective of the present study was to assess the cytotoxicity in cell cultures using different alginate materials for dental application and to verify the hypothesis that different alginate formulations promote different cell reactions. The cell culture was supplemented with 2 mM of L-glutamine Sigma, St. The total sample consisted of 14 dental alginates from different manufacturers and divided into 14 groups as follows: After adequate homogenization, the alginate was inserted into silicon rings 4 mm diameter x 4 mm height until full gelification Fig.
To verify the cell response to extreme situations, other 3 groups were included in the study: Assessing the Cytotoxity of the Materials. The materials were previously sterilized by exposing them to ultra-violet light Labconco, Kansas, Missouri, USA during 1 hour. The culture medium was replaced with fresh medium every 24 hours, and the supernatants were collected after 24, 48, 72, and hours 7 days for analysis of the toxicity to L cells.
After dek hour incubation period, ? Louis, Missouri, USA were added to the medium within each well of the plates, and these were incubated for 3 hours at 37oC to allow the dye to penetrate into the living cells. After this period, the cells were aalginato using ? Statistical analysis were performed by using a SPSS v. On the other hand, there were statistical differences between CC and C- groups in relation to other ones.
With regard to cell viability, Jeltrate Plus was the material showing the highest percentage of viable cells compared to other groups, followed by Hydrogum Alginate is undoubtedly one of the most accepted materials used in dentistry.
The alginate manufacturers produce a powder containing various compounds composicionn different aims. However, many substances such as zinc, barium, cadmium, lead silicates, and fluorides are added to their formulations in order to improve the physical, chemical, and mechanical properties, being a cause of concern in terms of toxicity de Freitas, That is, this substance may not be toxic enough to kill the cells but can inhibit cell growth, affecting at least the normal cell function.
The clinical meaning of this effect is that a single contact with the material may not cause clinical symptoms, whereas repeated contacts may affect the cell viability and consequently cause delayed allergic or toxic reaction. Therefore, the present work aimed at assessing the cytotoxicity of dental alginates in cell cultures. The use of cell cultures has been widely employed as part of a series of recommended tests to assess the biological behaviour of materials put in contact with human tissues Estrela, ; Jorge et al.
Alginato de sódio
In the present study, the cytotoxicity of dental alginates was assessed through cytotoxic tests performed with mouse fibroblast L cells, a method largely used in several works evaluating the cytotoxicity of materials for use in dentistry Alcaide et al. The two-minute evaluation adopted in this study was based on the maximum time compsicion which alginate is left in contact with alginwto oral mucosa during the impression procedure, as recommended by the manufacturer.
The samples were kept in contact with culture medium during this 2-minute period, and the supernatants were then put in contact with dejtal cells.
The samples were not placed directly upon the cells because mechanical contact between them might damage the cells as suggested by Costa et al. The results obtained showed that all alginate materials are cytotoxicity compared to the control groups CC and C.
Jeltrate Plus alginate was found to have the higher percentage of viable cells The other alginates had intermediate results. The lower cell cytotoxicity observed in the Jeltrate Plus alginate can be explained by the absence of lead in this material, a finding also corroborated composicioj Samuel et al.
Conversely, Samuel et al. The material used in the positive control group was a non-ionic surfactant Tweena toxic agent to biological membranes Rege et al. On the other hand, the negative control group used a PBS solution phosphate-buffered salinean acknowledged non-toxic agent, in order to assess only the physical effect on the cells as no other substance was put in contact with the cells.
As expected, a low cytotoxicity was observed and no statistical difference found. It should also be emphasized that a successful clinical dentistry does not involve the technique mastery only, but also requires application of biosecurity norms and attention to the local and systemic consequences produced by dental materials being used.
These possible cytotoxic effects should be evaluated in order to improve the security for a given dental material. In conclusions based on the results obtained, one can conclude that all alginate materials were found to be potentially toxic to cells. It is important to select low-cytotoxicity materials before acquiring new products. Rio de Janeiro, Guanabara Koogan S.
Quantitative analysis of potentially toxic metals in alginates for dental use. Cytotoxic effects of cleansing solutions recommended for chemical lavage of pulp exposures. Potential toxicants in alginate powders. The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C Cytotoxicity evaluation of Activ GP and Resilon cones in vitro. Cytotoxic effect of saffron stigma aqueous extract on human transitional cell carcinoma and mouse fibroblast.
Alginato de sódio – Wikipédia, a enciclopédia livre
Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells. Cytotoxicity of the dental materials. Cytotoxicity of dental alginates. Development of a model to demonstrate photosensitizer-mediated viral inactivation in blood. Effects of nonionic surfactants on membrane transporters in Caco-2 cell monolayers.
Porto Alegre, 36 2: Cytotoxicity of composixion orthodontic elastics. Interference of the detergent Tween 80 in protein assays. Cytotoxicity of impression materials.
Alginate, or irreversible hydrocolloid, is one of the most accepted impression materials used in dentistry. However, some substances existing in these materials can be toxic. The aim of this study was dep assess the cytotoxicity of alginates for dental applications. Fourteen different alginates were assessed: Three control groups were also used in this study: After contact with the medium, the cells were incubated for further 24 hours in which ?
Cells were incubated again for 3 hours so that the stain could be absorbed. After this period, the cells were fixed and viable cell counting was performed by using a spectrophotometer BioTek, Winooski, Vermont, USA at wavelength of nm. One can conclude, based on the results of this study, that all alginate materials were found alginaho be dentxl. Samples prepared prior to cytotoxicity assessment.