Jatropha gaumeri Greenm.; pomol che’. Tree (or shrub) with milky latex; leaves alternate, simple, broadly ovate, base cordate, margin entire, apex acuminate to. Jatropha gaumeri is a species of plants with 18 observations. Family: Euphorbiaceae Juss. Genus: Jatropha L. Jatropha gaumeri Greenm. This species is accepted, and its native range is SE. Mexico (and Tamaulipas) to.
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Isolation, structural identification and cytotoxic activity of hexanic extract, cyperenoic acid, and jatrophone terpenes from Jatropha ribifolia roots. Ruiz IV ; Mary A. Compounds jatrophone jatrpoha cyperenoic acid were isolated from the hexanic extract and characterized by spectroscopic techniques NMR of 1 H, 13 C and IR. The in vitro antiproliferative activity of jatrophone showed jatorpha in a concentration dependent way with Total Inhibition growth of: The latex is used by folkmedicine for treatment of snake bites and to treat jatopha tract decongestions.
Herein we report the isolation and structure elucidation of the compounds jatrophone and cyperenoic acid with in vitro antiproliferative activity. Voucher specimen CGMS The solvent was removed under reduced pressure yielding g The crude dried n -hexane-soluble fraction 7.
Fractions eluted with n -hexane: Cyperenoic acid was isolated from the crude n -hexane extract obtained from freshly picked roots g further extracted with n -hexane under reflux. After removal of the organic solvent under reduced pressure, 8 g of the extract was purified by silica gel column chromatography affording The active compounds were monitored by the in vitro antiproliferative assay. In vitro antiproliferative assay in human cancer jstropha lines. For the agumeri vitro antiproliferative activity screening, ten cell lines were selected from human tumors, designated as: The experimental procedures were performed according with the literature Skehan et al.
Stock solution of the n -hexanic extract, jatrophone 1 and cyperenoic acid 2 compounds mg mL -1 were prepared in dimethylsulfoxide DMSO. The supernatant was collected and discarded and precipitated of cells were resuspended in 5 mL culture medium. Jatro;ha proliferation was determined by spectrophotometric quantification at nm using the sulforhodamine B as dyeing reagent. The averages of the absorbance were calculated discounting the value the white and total growth inhibition TGI was determined by the equation: The absorbance data were analyzed and compiled in constructing graphs using the Origin 7.
Isolation and structural identification. The antitumor properties of species of the genus Jatropha have been targets of phytochemical studies. No previous reports on in vitro antiprolifrative activity on human cancer cell lines Jatropha ribifolia Pohl Baill.
Jatropha gaumeri Greenm.
The FTIR spectrum showed a broad absorption at cm -1 due to the presence of OH group and carbonyl group at cm -1 indicating the presence of carboxylic acid. This is the first time where the presence of this compound was described in this specie.
The NOESY spectrums of jatrophone and cyperenoic acid showed the H groups in proximity Figure 1 and 2 confirming the stereochemical aspects observed by Gaueri, for both compounds. In vitro antitumour activity. This is the first report exploring the in vitro antiproliferative activities effect of J. A range of J. Jatropah 3 shows the curves of the concentration response for the doxorrubicin control drug, hexanic extract, cyperenoic acid and jatrophone terpenes against human tumor cells.
Table 3 presents the results determined by the in vitro antiproliferative assay with human cancer cell lines. The activity of hexanic extract was efficient for seven cell lines tested, when compared with doxorrubicine, with an in vitro antiproliferative activity ranging from 1. The hexanic extract’s cytocidal activity against gaumedi cells growth was significantly efficient for seven strains.
The best selectivity was observed with glioma U with 1. The activity on tumoral cells was also observed for the crude hexane extract due to the presence of jatrophone and cyperenoic acid. The terpenes when isolated also showed significant results in the bioassay under study. The cytocidal activity for cyperenoic acid against tumoral growth cells was efficient for three tested cells.
The TGI for tumoral cells were For VERO cells 9. Better results were noted for jatrophone 1 with selectivity in a concentration dependent way determined as 0. Further studies are necessary to better understand the toxic effects toward normal cells. These terpenes were isolated in other Euphorbiaceae species Pertino et al.
Bioactive Terpenoids from Roots and Leaves of Jatropha gaumeri
Previous authors evaluated activities against epithelial gastric cell line AGS and gastroprotecion effect Pertino et al. Cyperenoic acid 2 has been also isolated from different species of plants as Sandwithia guyanensis Jacobs et al. Jatroopha preliminary results presented here show that jatrophone 1 and cyperenoic acid 2 could be a promising molecule to study for the development of a new cancer treatment.
The authors are grateful to Dr. Barata and the University of Campinas for the chemical analysis. ESF, FAR, and DT graduation students contributed in gaumerk plant sample and identification and isolation, purification of the extracts of the jagropha compounds. PMI contributed to purification and spectroscopic analysis. All the authors have read the final manuscript and gaumfri the submission. X-ray structure of cyperenoic acid from Croton crassifolius. Bioactive terpenoids from roots and leaves of Jatropha gaumeri.
Rev Soc Quim Mex Applications of 2D-NMR spectroscopy to phytochemical studies: J Nat Prod Feasibility of a high flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer I Gastroprotective activity and cytotoxic effect of cyperenoic acid derivatives.
J Jatroha Pharmacol Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. New colorimetric cytotoxicity assay for anticancer-drug screening. New Antileukemic jatrophone derivatives from Jatropha gossypiifolia: J Am Chem Soc Terpenoids as therapeutic drugs and pharmaceutical agents.
Zhang L, Demain L eds. Drug Discovery and Therapeutic Medicine. Received 18 Sep Accepted 23 Jan All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.
Services on Demand Journal. Isolation procedures Fresh roots 1. Biological tests In vitro antiproliferative assay in human cancer cell lines For the in vitro antiproliferative activity screening, ten cell lines were jattopha from human tumors, designated as: Date analysis The averages of the absorbance were calculated discounting the value the white and total growth inhibition TGI was determined by the equation: Results and Discussion Isolation and structural identification The antitumor properties of species of the genus Jatropha have been targets of phytochemical studies.
In vitro antitumour activity This is the first report exploring the in vitro antiproliferative activities effect of J. Acknowledgment The authors are grateful to Dr. Authors’ contributions ESF, FAR, and DT graduation students contributed in collecting plant sample and identification and isolation, purification of the extracts of the isolated compounds.
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